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More recently, increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The expressed nucleases then knock out and replace genes in the chromosome.As of 2014 these approaches involve removing cells from patients, editing a chromosome and returning the transformed cells to patients.Aside from their historical interest, it is often worth knowing about older technology, as it is occasionally useful to solve another new problem for which the newer technique is inappropriate rapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease.The first attempt at modifying human DNA was performed in 1980 by Martin Cline, but the first successful and approved nuclear gene transfer in humans was performed in May 1989.Short (20-25 nucleotides in length), labeled probes are exposed to the non-fragmented target DNA, hybridization occurs with high specificity due to the short length of the probes and even a single base change will hinder hybridization.The target DNA is then washed and the labeled probes that didn't hybridize are removed.PCR can also be used to determine whether a particular DNA fragment is found in a c DNA library.

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Molecular biology concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, and proteins and their biosynthesis, as well as the regulation of these interactions.

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The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September 1990.

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